The 53rd In Silico Megabank Research Seminar will be held on Friday, January 9.

This Time, we will be welcoming Dr. Tomoko Shibata, The National Institute for Basic Biology as our lecturer, and she will be speaking on “Development of a New Genomic Analysis Method Using Single Module Sequencing from Small Quantities of DNA”.

・Date/Time: January 9 (Friday) 9:00‐10:15
・Venue: Small Conference Room 2(3rd Floor), Tohoku Medical Megabank Building
・Title: Development of a New Genomic Analysis Method Using Single Module Sequencing from Small Quantities of DNA
・Lecturer: Tomoko Shibata (The National Institute for Basic Biology )

・Abstract: Single Module Sequencer, PacBio RSII, has a long average length of readable sequence of 7−8 kb with maximum of 30 kb, and it is possible to determine the base sequence without the amplification through PCR during the process of sequencing. Thus, domains with difficulty in amplification and long repetitive sequences became readable, which enabled more exhaustive genomic analysis. However, on the contrary to the unnecessity of amplification, large quantities of DNA are required for analyses. Moreover, in many cases sufficient amount of DNA cannot be secured depending on living organisms. Hence, we aimed to develop a method which enables genomic analyses with small quantities of DNA by using PacBio sequencer on DNA amplified with Phi29 DNA polymerase which is known to amplify DNA without causing a bias. In this seminar, we will introduce the results of the data analysis of bacterial genome and eukaryotic genome and discuss the future prospects.

・Organizer: Masao Nagasaki